ITGA4 Antikörper (FITC)

Produktnummer ABIN3044633

Produktdetails anti-ITGA4 Antikörper (FITC)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser ITGA4 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Produktmerkmale: Monoclonal Anti-Mouse CD49d is recommended for use in flow cytometry for identification of .

Immunogen: AKR/Cum mouse spontaneous T lymphoma line TK1 Species

Klon: R1-2

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using 1 µg/10^6 cells

Kommentare:

Fluorescein Isothiocyanate, FITC (Abs/Em: 495/ 517 nm).

Aufbereitung der Reagenzien:

1. Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse CD49d FITC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Lagerung: 4 °C

Details zu ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Andere Bezeichnung: CD49d (ITGA4 Produkte)

Synonyme: CD49D antikoerper, IA4 antikoerper, LOC100219853 antikoerper, integrin subunit alpha 4 antikoerper, integrin alpha 4 antikoerper, integrin subunit alpha 4 S homeolog antikoerper, ITGA4 antikoerper, Itga4 antikoerper, itga4.S antikoerper

Hintergrund: CD49d is a 150 kDa glycoprotein, also known as alpha4 integrin or VLA-4 alpha chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells.

Pathways: Integrin Complex