BUB3 Antikörper (Middle Region)

Produktnummer ABIN3044239

Produktdetails anti-BUB3 Antikörper

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Bindungsspezifität: AA 76-90, Middle Region

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BUB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Mitotic checkpoint protein BUB3(BUB3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequenz: DHQLKMHDLN TDQEN

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Mitotic checkpoint protein BUB3(BUB3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: budding uninhibited by benzimidazoles 3 homolog(yeast)
Protein Name: Mitotic checkpoint protein BUB3

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Bub3(76-90aa DHQLKMHDLNTDQEN), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Andere Bezeichnung: BUB3 (BUB3 Produkte)

Synonyme: BUB3L antikoerper, hBUB3 antikoerper, Aa2-050 antikoerper, xbub3 antikoerper, AU019800 antikoerper, AU021329 antikoerper, AU043350 antikoerper, AW146323 antikoerper, C78067 antikoerper, BUB3, mitotic checkpoint protein antikoerper, BUB3 mitotic checkpoint protein antikoerper, BUB3 mitotic checkpoint protein L homeolog antikoerper, BUB3 antikoerper, Bub3 antikoerper, bub3.L antikoerper

Hintergrund: BUB3(BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint(SAC), though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.

Synonyms: Aa2-050 antibody|AU019800 antibody|AU021329 antibody|AU043350 antibody|AW146323 antibody|BUB 3 antibody|BUB 3L antibody|BUB3(budding uninhibited by benzimidazoles 3, yeast) homolog antibody|bub3 antibody|BUB3 budding uninhibited by benzimidazoles 3 homolog antibody|BUB3_HUMAN antibody|BUB3L antibody|Budding uninhibited by benomyl antibody|Budding uninhibited by benzimidazoles 3 antibody|Budding uninhibited by benzimidazoles 3 homolog(S. cerevisiae) antibody|Budding uninhibited by benzimidazoles 3 homolog(yeast) antibody|Budding uninhibited by benzimidazoles 3 homolog antibody|Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody|C78067 antibody|hBUB3 antibody|Mitotic checkpoint component antibody|Mitotic checkpoint protein bub3 antibody|OTTHUMP00000020679 antibody|OTTHUMP00000020680 antibody|WD repeat type I transmembrane protein A72.5 antibody

UniProt: O43684

Pathways: Maintenance of Protein Location