HSP70 1A Antikörper (C-Term)

Produktnummer ABIN3044190

Produktdetails anti-HSP70 1A Antikörper

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Bindungsspezifität: AA 577-596, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSP70 1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Heat shock 70 kDa protein 1A/1B(HSPA1A/HSPA1B) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Rat.

Sequenz: VISWLDANTL AEKDEFEHKR

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Heat shock 70 kDa protein 1A/1B(HSPA1A/HSPA1B) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Rat.
Gene Name: heat shock protein family A (Hsp70) member 1A/1B
Protein Name: Heat shock 70 kDa protein 1A/1B

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70(577-596aa VISWLDANTLAEKDEFEHKR), different from the related mouse sequence by four amino acids and from the related rat sequence by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-HSP70 1A Antikörper (ABIN3044190)

Zhang, Tang, Li, Li, Zhang, Yan, Cui: "Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5371-8, (2016) (PubMed).

Chen, Wan, Huang, Wang, Liu, Wang: "Study on the immune response to recombinant Hsp70 protein from Megalobrama amblycephala." in: Immunobiology, Vol. 219, Issue 11, pp. 850-8, (2014) (PubMed).

Jing, Liu, Chen, Hu, Xiao, Ma: "Acute effect of copper and cadmium exposure on the expression of heat shock protein 70 in the Cyprinidae fish Tanichthys albonubes." in: Chemosphere, Vol. 91, Issue 8, pp. 1113-22, (2013) (PubMed).

Pu, Shi, Wang, Wang, Guo, Liu, Sun, Gao, Zhou: "Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats." in: Journal of biomedical research, Vol. 25, Issue 4, pp. 292-8, (2013) (PubMed).

Chen, Zuo, Yang, Lu, Wang, Wang, Tu, Chen, Liu, Liu, Xiao: "Severe multiple organ injury in HSF1 knockout mice induced by lipopolysaccharide is associated with an increase in neutrophil infiltration and surface expression of adhesion molecules." in: Journal of leukocyte biology, Vol. 92, Issue 4, pp. 851-7, (2012) (PubMed).

Li, Roubeix, Wang, Shi, Liu, Baudouin, Chen: "Therapeutic efficacy of trehalose eye drops for treatment of murine dry eye induced by an intelligently controlled environmental system." in: Molecular vision, Vol. 18, pp. 317-29, (2012) (PubMed).

Fan, Jiang, Yang, Liu, Song, Pan: "Effects of adrenergic agents on stress-induced brain microstructural and immunochemical changes in adult male Wistar rats." in: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, Vol. 193, Issue 5, pp. 418-24, (2011) (PubMed).

Xu, Luo, Wu, Chen, Xu, Gao, He, Geng, Xiao, Zhang, Chen, Li: "Immunohistological evaluation of skin responses after treatment using a fractional ultrapulse carbon dioxide laser on back skin." in: Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], Vol. 37, Issue 8, pp. 1141-9, (2011) (PubMed).

Ouyang, Gao, Wang, Xie, Lei, Zhou, Liao, Zhong, Tang: "Thermoseed hyperthermia treatment of mammary orthotopic transplantation tumors in rats and impact on immune function." in: Oncology reports, Vol. 24, Issue 4, pp. 973-9, (2010) (PubMed).

Yi, Peng, Chang, Peng, Yan, Lin: "Effect of pre-moxibustion on apoptosis and proliferation of gastric mucosa cells." in: World journal of gastroenterology, Vol. 13, Issue 15, pp. 2174-8, (2007) (PubMed).

Wu, Cao, Gao, Chen, Wang, Zumbika, Qian: "Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells." in: World journal of gastroenterology, Vol. 10, Issue 20, pp. 2944-8, (2004) (PubMed).

Details zu HSP70 1A

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Andere Bezeichnung: HSPA1A (HSPA1A Produkte)

Synonyme: hsp70-4 antikoerper, hspa1a antikoerper, Hsp70-3 antikoerper, Hsp70.3 antikoerper, Hsp72 antikoerper, hsp68 antikoerper, hsp70A1 antikoerper, HSP72 antikoerper, Hsp70-1 antikoerper, Hspa1 antikoerper, Hspa1b antikoerper, hsp70-1 antikoerper, hsp70-1a antikoerper, hsp70i antikoerper, hsp72 antikoerper, hspa1 antikoerper, hspa1b antikoerper, HSP70-1 antikoerper, HSP70-1A antikoerper, HSP70I antikoerper, HSPA1 antikoerper, HSPA1A antikoerper, HSPA1B antikoerper, HSP70-2 antikoerper, HSPA2 antikoerper, HSP70 antikoerper, hspA1A antikoerper, heat shock cognate 70-kd protein, tandem duplicate 3 antikoerper, heat shock 70 kDa protein 1 antikoerper, heat shock 70 kDa protein II antikoerper, heat shock protein 1A antikoerper, heat shock 70kD protein 1A antikoerper, heat shock protein family A (Hsp70) member 1A S homeolog antikoerper, heat shock protein family A (Hsp70) member 1A antikoerper, heat shock protein 70.2 antikoerper, heat shock 70kDa protein 1A antikoerper, heat shock 70 kDa protein 1B antikoerper, heat shock protein 70.1 antikoerper, hsp70.3 antikoerper, LOC100023597 antikoerper, LOC100554002 antikoerper, LOC100608888 antikoerper, Hspa1a antikoerper, hspa1a.S antikoerper, HSPA1A antikoerper, HSP70.2 antikoerper, LOC100354037 antikoerper, HSP70.1 antikoerper

Hintergrund: HSPA1(heat shock 70 kDa protein 1A) also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. The HSPA1 gene is mapped on 6p21.33. Shimizu et al.(1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.

Synonyms: DAQB 147D11.1 001 antibody|FLJ54303 antibody|FLJ54370 antibody|FLJ54392 antibody|FLJ54408 antibody|FLJ75127 antibody|Heat shock 70 kDa protein 1 antibody|Heat shock 70 kDa protein 1/2 antibody|Heat shock 70 kDa protein 1A/1B antibody|heat shock 70 kDa protein 1A antibody|Heat shock 70 kDa protein 1B antibody|Heat shock induced protein antibody|heat shock protein 70 antibody|HSP70 1 antibody|HSP70 2 antibody|HSP70-1/HSP70-2 antibody|HSP70-1A antibody|HSP70.1 antibody|HSP70.1/HSP70.2 antibody|HSP70I antibody|HSP71_HUMAN antibody|HSP72 antibody|HSPA1 antibody|HSPA1A antibody|HSPA1B antibody|XXbac BCX40G17.3 001 antibody

UniProt: P08P09

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process