RAB5 Antikörper (C-Term)

Produktnummer ABIN3043917

Produktdetails anti-RAB5 Antikörper

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Bindungsspezifität: AA 179-215, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAB5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Ras-related protein Rab-5A(RAB5A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: KKLPKNEPQN PGANSARGRG VDLTEPTQPT RNQCCSN

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Ras-related protein Rab-5A(RAB5A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: RAB5A, member RAS oncogene family
Protein Name: Ras-related protein Rab-5A

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5 (179-215aa KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN), different from the related mouse and rat sequences by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-RAB5 Antikörper (ABIN3043917)

Han, Jiang, Ding, Liu, Xiao, Shi: "Change of Rin1 and Stathmin in the Animal Model of Traumatic Stresses." in: Frontiers in behavioral neuroscience, Vol. 11, pp. 62, (2017) (PubMed).

Details zu RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Andere Bezeichnung: RAB5A (RAB5A Produkte)

Synonyme: RAB5 antikoerper, 2410015H04Rik antikoerper, AI663973 antikoerper, AU021172 antikoerper, nnyRab5a antikoerper, DDBDRAFT_0168594 antikoerper, DDBDRAFT_0229401 antikoerper, DDB_0168594 antikoerper, DDB_0229401 antikoerper, rab5 antikoerper, RAB5A antikoerper, Rab5a antikoerper, rab5al antikoerper, zgc:56644 antikoerper, fj38g08 antikoerper, hm:zehn1144 antikoerper, rab5a antikoerper, wu:fj38g08 antikoerper, RAB5A, member RAS oncogene family antikoerper, rab5A protein antikoerper, Rab5a, GTPase antikoerper, predicted protein antikoerper, Rab GTPase antikoerper, RAB5A, member RAS oncogene family L homeolog antikoerper, RAB5A, member RAS oncogene family, b antikoerper, RAB5A, member RAS oncogene family, a antikoerper, RAB5A antikoerper, Rab5a antikoerper, rab5A antikoerper, rab5a antikoerper, rab5a.L antikoerper, rab5ab antikoerper, rab5aa antikoerper

Hintergrund: RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.

Synonyms: RAB 5 antibody|RAB 5A antibody|RAB5A antibody|RAB5A member RAS oncogene family antibody|RAB5A_HUMAN antibody|RAS associated protein RAB5A antibody|Ras related protein Rab 5A antibody|Ras-related protein Rab-5A antibody

Gen-ID: 5868

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity