Actin Antikörper (C-Term)

Produktnummer ABIN3043518

Produktdetails anti-Actin Antikörper

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Bindungsspezifität: AA 359-377, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Actin, alpha skeletal muscle(ACTA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: ITKQEYDEAG PSIVHRKCF

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Actin, alpha skeletal muscle(ACTA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: actin, alpha 1, skeletal muscle
Protein Name: Actin, alpha skeletal muscle

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (359-377aa ITKQEYDEAGPSIVHRKCF), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Actin is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-Actin Antikörper (ABIN3043518)

Cui, Wang, Yu, Li, Xie, Gao, Wang, Zhu: "Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway." in: Molecular medicine reports, Vol. 14, Issue 2, pp. 1610-6, (2017) (PubMed).

Yan, Chen, Gong, Yin, Zhou, Chaugai, Wang: "miR-21-3p regulates cardiac hypertrophic response by targeting histone deacetylase-8." in: Cardiovascular research, Vol. 105, Issue 3, pp. 340-52, (2015) (PubMed).

Mitsuhashi, Shindo, Shigetomi, Miyamoto, Ubukata: "(+)-Epogymnolactam, a novel autophagy inducer from mycelial culture of Gymnopus sp." in: Phytochemistry, Vol. 114, pp. 163-7, (2015) (PubMed).

Wu, Sun, Wu, Li, Du, Zeng, Bi, Sun: "Activation of calcium-sensing receptor increases TRPC3/6 expression in T lymphocyte in sepsis." in: Molecular immunology, Vol. 64, Issue 1, pp. 18-25, (2015) (PubMed).

Hong, Zhang, Zhang, Yang, Wan, Yu, Wang, Feng, Li, Yew: "Expression of dopamine transporter in the different cerebral regions of methamphetamine-dependent rats." in: Human & experimental toxicology, Vol. 34, Issue 7, pp. 707-17, (2015) (PubMed).

Xie, Zhang, Chen, Pan, Wu, Ge, Yang: "Effects of ghrelin on postresuscitation brain injury in a rat model of cardiac arrest." in: Shock (Augusta, Ga.), Vol. 43, Issue 5, pp. 490-6, (2015) (PubMed).

Dai, Yin, Zhao, Guo, Li, Ma, Lu: "III-10, a newly synthesized flavonoid, induces cell apoptosis with the involvement of reactive oxygen species-mitochondria pathway in human hepatocellular carcinoma cells." in: European journal of pharmacology, Vol. 764, pp. 353-62, (2015) (PubMed).

Cheng, Ji, Liu, Mei, Wang, Feng, Ding: "Erratum: Expression changes of ionic channels in early phase of cultured rat atrial myocytes induced by rapid pacing." in: Journal of cardiothoracic surgery, Vol. 10, pp. 10, (2015) (PubMed).

Liu, Wang, Wang, Wang: "Anticancer effects of chemokine receptor 4(CXCR4) gene silenced by CXCR4-siRNA in nude mice model of ovarian cancer." in: Cell biochemistry and biophysics, Vol. 70, Issue 3, pp. 1893-900, (2014) (PubMed).

Chen, Yang, Pang, Peng, Dong, Liu, Ouyang, Ren: "Expression, purification and antibody preparation using different constructs of PCV2 capsid protein." in: International journal of biological macromolecules, Vol. 67, pp. 289-94, (2014) (PubMed).

Gong, Liu, Ding, Liu, Li, Zheng, Jia, Zhou, Zou, Li, Huang, Fan: "Physiological pulsatile flow culture conditions to generate functional endothelium on a sulfated silk fibroin nanofibrous scaffold." in: Biomaterials, Vol. 35, Issue 17, pp. 4782-91, (2014) (PubMed).

Tao, Fu, Wang, Yao, Zhou, Dai, Li, Lu, Wang: "LL-202, a newly synthesized flavonoid, inhibits tumor growth via inducing G(2)/M phase arrest and cell apoptosis in MCF-7 human breast cancer cells in vitro and in vivo." in: Toxicology letters, Vol. 228, Issue 1, pp. 1-12, (2014) (PubMed).

Li, Chen, Ouyang, Chen, Wu, Kuang, Zheng: "Increased autophagy sustains the survival and pro-tumourigenic effects of neutrophils in human hepatocellular carcinoma." in: Journal of hepatology, Vol. 62, Issue 1, pp. 131-9, (2014) (PubMed).

Zhou, Wang, Tokunaga, Huang, Sun, Yang: "The HIV-1 accessory protein Vpr induces the degradation of the anti-HIV-1 agent APOBEC3G through a VprBP-mediated proteasomal pathway." in: Virus research, Vol. 195, pp. 25-34, (2014) (PubMed).

Tang, Liu, Yang, Xie, Ye, Wu, Liu, Chen, Zhang, Xie: "miR-185 suppresses tumor proliferation by directly targeting E2F6 and DNMT1 and indirectly upregulating BRCA1 in triple-negative breast cancer." in: Molecular cancer therapeutics, Vol. 13, Issue 12, pp. 3185-97, (2014) (PubMed).

Cheng, Zhao, Wang, Lu, Wang, Wang, Yao: "The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray." in: International journal of molecular sciences, Vol. 15, Issue 1, pp. 186-202, (2014) (PubMed).

Li, Chen, Li, Zhang, Tang: "Cytotoxicity of polyaniline nanomaterial on rat celiac macrophages in vitro." in: PLoS ONE, Vol. 9, Issue 9, pp. e107361, (2014) (PubMed).

Li, Shang: "Inhaled corticosteroids inhibit substance P receptor expression in asthmatic rat airway smooth muscle cells." in: BMC pulmonary medicine, Vol. 12, pp. 79, (2013) (PubMed).

Yu, Dai, Chen, Zang, Deng, Liu, Ying: "Hypolipidemic and antioxidant activities of polysaccharides from Rosae Laevigatae Fructus in rats." in: Carbohydrate polymers, Vol. 94, Issue 1, pp. 56-62, (2013) (PubMed).

Leng, Wang, Pang, Zhan, Zhang, Wang, Gao, Qian: "Evidence of a role for both anti-Hsp70 antibody and endothelial surface membrane Hsp70 in atherosclerosis." in: Cell stress & chaperones, Vol. 18, Issue 4, pp. 483-93, (2013) (PubMed).

Details zu Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Andere Bezeichnung: ACTA1 (ACTA1 Produkte)

Synonyme: ACTA antikoerper, ASMA antikoerper, CFTD antikoerper, CFTD1 antikoerper, CFTDM antikoerper, MPFD antikoerper, NEM1 antikoerper, NEM2 antikoerper, NEM3 antikoerper, acta antikoerper, asma antikoerper, cftd antikoerper, cftd1 antikoerper, cftdm antikoerper, mpfd antikoerper, nem1 antikoerper, AA959943 antikoerper, Acta-2 antikoerper, Acts antikoerper, Actsk-1 antikoerper, actc antikoerper, actc1 antikoerper, cfk antikoerper, hm:zeh0631 antikoerper, zeh0631 antikoerper, acta1 antikoerper, hm:zeh0293 antikoerper, zgc:112098 antikoerper, actin, alpha 1, skeletal muscle antikoerper, actin, alpha 1, skeletal muscle L homeolog antikoerper, actin, alpha 1b, skeletal muscle antikoerper, actin, muscle antikoerper, actin, alpha skeletal muscle antikoerper, actin, alpha 1a, skeletal muscle antikoerper, ACTA1 antikoerper, acta1.L antikoerper, acta1 antikoerper, Acta1 antikoerper, acta1b antikoerper, LOC100533357 antikoerper, LOC109479779 antikoerper, LOC109108280 antikoerper, acta1a antikoerper

Hintergrund: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Synonyms: ACTA antibody|ACTA1 antibody|Actin alpha skeletal muscle antibody|Actin antibody|actin, alpha 1, skeletal muscle 1 antibody|actin, alpha 1, skeletal muscle antibody|Actin, alpha skeletal muscle antibody|actina antibody|actine antibody|ACTS_HUMAN antibody|aktin antibody|Alpha Actin 1 antibody|Alpha skeletal muscle Actin antibody|alpha skeletal muscle antibody|alpha-actin antibody|Alpha-actin-1 antibody|ASMA antibody|CFTD antibody| CFTD1antibody|CFTDM antibody|MPFD antibody|NEM1 antibody|NEM2 antibody|NEM3 antibody| nemaline myopathy type 3 antibody

UniProt: P68133

Pathways: Caspase Kaskade in der Apoptose, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development