Osteopontin Antikörper (N-Term)
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- Target Alle Osteopontin (SPP1) Antikörper anzeigen
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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Bindungsspezifität
- AA 28-47, N-Term
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Osteopontin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Verwendungszweck
- Rabbit IgG polyclonal antibody for Osteopontin(SPP1) detection. Tested with WB, IHC-P in Human.
- Sequenz
- EEKQLYNKYP DAVATWLNPD
- Kreuzreaktivität (Details)
- No cross reactivity with other proteins.
- Produktmerkmale
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Rabbit IgG polyclonal antibody for Osteopontin(SPP1) detection. Tested with WB, IHC-P in Human.
Gene Name: secreted phosphoprotein 1
Protein Name: Osteopontin - Aufreinigung
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human Osteopontin(28-47aa EEKQLYNKYPDAVATWLNPD), different from the related mouse sequence by six amino acids.
- Isotyp
- IgG
- Top Product
- Discover our top product SPP1 Primärantikörper
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- Applikationshinweise
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Konservierungsmittel
- Thimerosal (Merthiolate), Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Haltbarkeit
- 12 months
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Predictive value of stathmin-1 and osteopontin expression for taxan resistance in metastatic castrate-resistant prostate cancer." in: Pakistan journal of medical sciences, Vol. 33, Issue 3, pp. 560-565, (2017) (PubMed).
: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." in: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).
: "Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of ..." in: PLoS ONE, Vol. 11, Issue 7, pp. e0157456, (2016) (PubMed).
: "Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo." in: Stem cells international, Vol. 2015, pp. 758706, (2015) (PubMed).
: "Expression of both matrix metalloproteinase-2 and its tissue inhibitor-2 in tunica media of radial artery in uremic patients." in: Renal failure, Vol. 35, Issue 1, pp. 37-42, (2013) (PubMed).
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Predictive value of stathmin-1 and osteopontin expression for taxan resistance in metastatic castrate-resistant prostate cancer." in: Pakistan journal of medical sciences, Vol. 33, Issue 3, pp. 560-565, (2017) (PubMed).
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- Target
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- Andere Bezeichnung
- SPP1 (SPP1 Produkte)
- Synonyme
- BNSP antikoerper, BSPI antikoerper, ETA-1 antikoerper, OPN antikoerper, 2AR antikoerper, Apl-1 antikoerper, Bsp antikoerper, Eta antikoerper, OP antikoerper, Opn antikoerper, Opnl antikoerper, Ric antikoerper, Spp-1 antikoerper, OSP antikoerper, zgc:111821 antikoerper, secreted phosphoprotein 1 antikoerper, SPP1 antikoerper, Spp1 antikoerper, spp1 antikoerper
- Hintergrund
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Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.
Synonyms: BNSP antibody|Bone sialoprotein 1 antibody|BSP I antibody|BSPI antibody|Early T lymphocyte activation 1 antibody|ETA 1 antibody|ETA1 antibody|MGC110940 antibody|Nephropontin antibody|OPN antibody|Osteopontin antibody|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody|OSTP_HUMAN antibody|PSEC0156 antibody|secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody|Secreted phosphoprotein 1 antibody|SPP 1 antibody|SPP-1 antibody|SPP1 antibody|SPP1/CALPHA1 fusion antibody|Urinary stone protein antibody|Uropontin antibody - UniProt
- P10451
- Pathways
- Regulation of Cell Size
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