PAD4 Antikörper (Middle Region)

Produktnummer ABIN3032172

Produktdetails anti-PAD4 Antikörper

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Bindungsspezifität: Middle Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human PAD4 (ASPRSCYKLFQEQQNEGHGEA) was used as the immunogen for this PAD4 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the PAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the PAD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu PAD4

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Andere Bezeichnung: PAD4 (PADI4 Produkte)

Synonyme: PAD antikoerper, PAD4 antikoerper, PADI5 antikoerper, PDI4 antikoerper, PDI5 antikoerper, Pad4 antikoerper, Pdi4 antikoerper, peptidyl arginine deiminase 4 antikoerper, peptidyl arginine deiminase, type IV antikoerper, PADI4 antikoerper, Padi4 antikoerper

Hintergrund: Peptidylarginine Deiminase, Type IV is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion(Nakashima et al., 1999). Suzuki et al.(2003) found from sequence data that the gene maps to 1p36. Cuthbert et al.(2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that the protein specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PAD4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PAD4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that the protein could repress hormone receptor-mediated gene induction. It was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role.

UniProt: Q9UM07