AQP3 Antikörper (C-Term)

Produktnummer ABIN3030005

Produktdetails anti-AQP3 Antikörper

Target: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AQP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Aquaporin 3 (EENVKLAHVKHKEQI) was used as the immunogen for this AQP3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the AQP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the AQP3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu AQP3

Target: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Andere Bezeichnung: AQP3 Aquaporin 3 (AQP3 Produkte)

Synonyme: aqp3 antikoerper, wu:fa95h06 antikoerper, wu:fi13e05 antikoerper, zgc:55525 antikoerper, Tb06.28P18.240 antikoerper, AQP-2 antikoerper, AQP-3 antikoerper, GIL antikoerper, SAQP3 antikoerper, aquaporin 3a antikoerper, aquaporin 3 (Gill blood group) antikoerper, aquaporin 3 antikoerper, aqp3a antikoerper, AQP3 antikoerper, Tb927.6.1520 antikoerper, MCYG_07750 antikoerper, Aqp3 antikoerper

Hintergrund: Aquaporin 3 is the third member of the major intrinsic protein family. The protein is localized at the basal lateral membranes of collecting duct cells in the kidney. AQP3 appears to exist as a single copy and to comprise 6 exons distributed over 7 kb. The gene is mapped to 9p21-p12 by fluorescence in situ hybridization. The protein works as water-channel and facilitates the transport of nonionic small solutes such as urea and glycerol, albeit to a smaller degree. The results suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. By Western blot analysis and immunofluorescence microscopy of epidermis, Sougrat et al.(2002) showed strong expression of an approximately 33-kD glycosylated AQP3 protein in keratinocyte plasma membranes, one layer below the unstained stratum corneum(SC). It provides a short circuit for water, or water-clamp, between the base of the epidermis and the SC in order to maintain a constant H2O content and to prevent the formation of a continuous water gradient across the epidermis below the SC.

UniProt: Q92482