UCHL1 Antikörper

Produktnummer ABIN3026777

Produktdetails anti-UCHL1 Antikörper

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser UCHL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktmerkmale: This antibody specifically reacts with a protein of 20-30 kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. PGP9.5 antibody has been used to show the protein to be a useful prognostic indicator of colorectal, pancreatic and gall bladder cancer. A mutation in the gene is believed to cause a form of Parkinson's disease.

Aufreinigung: Protein G affinity chromatography

Immunogen: Native protein from brain was used as the immunogen for this PGP9.5 antibody.

Klon: 31A3

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PGP9.5 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the PGP9.5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Andere Bezeichnung: PGP9.5 UchL1 (UCHL1 Produkte)

Synonyme: PARK5 antikoerper, PGP 9.5 antikoerper, PGP9.5 antikoerper, PGP95 antikoerper, Uch-L1 antikoerper, UCH-L1 antikoerper, UCHL1 antikoerper, cb358 antikoerper, wu:fc55h08 antikoerper, park5 antikoerper, pgp9.5 antikoerper, uch-l1 antikoerper, MGC132191 antikoerper, uchl1 antikoerper, AW822034 antikoerper, C88048 antikoerper, R75593 antikoerper, UCHL-1 antikoerper, gad antikoerper, ubiquitin C-terminal hydrolase L1 antikoerper, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) antikoerper, ubiquitin C-terminal hydrolase L1 L homeolog antikoerper, ubiquitin carboxyl-terminal esterase L1 antikoerper, ubiquitin carboxy-terminal hydrolase L1 antikoerper, UCHL1 antikoerper, uchl1 antikoerper, uchl1.L antikoerper, LOC100304750 antikoerper, Uchl1 antikoerper

Hintergrund: This antibody specifically reacts with a protein of 20-30  kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. PGP9.5 antibody has been used to show the protein to be a useful prognostic indicator of colorectal, pancreatic and gall bladder cancer. A mutation in the gene is believed to cause a form of Parkinson's disease.

Gen-ID: 7345

Pathways: Feeding Behaviour