KRT1 Antikörper
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- Target Alle KRT1 Antikörper anzeigen
- KRT1 (Cytokeratin HMW (KRT1))
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Reaktivität
- Human, Ratte, Maus
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser KRT1 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Produktmerkmale
- This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.
- Klon
- 34BE12
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product KRT1 Primärantikörper
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- Applikationshinweise
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HMW Cytokeratin antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2) - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store the HMW Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- KRT1 (Cytokeratin HMW (KRT1))
- Andere Bezeichnung
- HMW Cytokeratin (KRT1 Produkte)
- Synonyme
- CK1 antikoerper, EHK antikoerper, EHK1 antikoerper, EPPK antikoerper, K1 antikoerper, KRT1A antikoerper, NEPPK antikoerper, keratin 1 antikoerper, KRT1 antikoerper
- Hintergrund
- This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.
- Gen-ID
- 3848
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