TYMS Antikörper

Produktnummer ABIN3026351

Produktdetails anti-TYMS Antikörper

Target: TYMS (Thymidylate Synthetase (TYMS))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TYMS Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktmerkmale: This antibody is specific for a protein of 36 kDa, identified as Thymidylate Synthase (TS). Thymidylate synthase converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. Thymidylate synthase is also a critical target for the fluoropyrimidines, an important group of anti-neoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates thymidylate synthase by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of thymidylate synthase protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Aufreinigung: Protein G purified monoclonal antibody

Immunogen: Recombinant human Thymidylate synthase was used as the immunogen for this antibody.

Klon: TMS715

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Thymidylate Synthase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Andere Bezeichnung: Thymidylate Synthase (TYMS Produkte)

Synonyme: FN1 antikoerper, ECK2823 antikoerper, JW2795 antikoerper, ts antikoerper, Ts antikoerper, HST422 antikoerper, TMS antikoerper, TS antikoerper, thymidylate synthetase antikoerper, contains intron in T4 phage antikoerper, ThyE antikoerper, thymidylate synthase antikoerper, thymidylate synthetase L homeolog antikoerper, TYMS antikoerper, thyA antikoerper, td antikoerper, thyE antikoerper, DDA3937_RS04960 antikoerper, tyms.L antikoerper, XBJ1_RS15770 antikoerper, EAMY_RS20680 antikoerper, Tyms antikoerper

Substanzklasse: Viral Protein

Hintergrund: This antibody is specific for a protein of 36  kDa, identified as Thymidylate Synthase (TS). Thymidylate synthase converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. Thymidylate synthase is also a critical target for the fluoropyrimidines, an important group of anti-neoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates thymidylate synthase by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of thymidylate synthase protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Gen-ID: 7298

Pathways: Mitotic G1-G1/S Phases