CD99 Antikörper

Produktnummer ABIN3025987

Produktdetails anti-CD99 Antikörper

Target: CD99

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD99 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Keine Kreuzreaktivität: Ratte (Rattus)

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the CD99 antibody.

Klon: MIC2-877

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CD99 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD99

Target: CD99

Andere Bezeichnung: CD99 (CD99 Produkte)

Synonyme: 1110061M03Rik antikoerper, 2410026K10Rik antikoerper, D4 antikoerper, Pilr-l antikoerper, pilr-1 antikoerper, HBA71 antikoerper, MIC2 antikoerper, MIC2X antikoerper, MIC2Y antikoerper, MSK5X antikoerper, CD99 antigen antikoerper, CD99 molecule (Xg blood group) antikoerper, CD99 molecule antikoerper, Cd99 antikoerper, CD99 antikoerper

Substanzklasse: Viral Protein

Hintergrund: Recognizes a sialoglycoprotein of 27-32  kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32  kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. It is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.