MAML3 Antikörper

Produktnummer ABIN3025220

Produktdetails anti-MAML3 Antikörper

Target: MAML3 (Mastermind-Like 3 (MAML3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAML3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: A human full length recombinant protein was used as the immunogen for this MAML3 antibody.

Klon: MMLP3-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML3 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the MAML3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu MAML3

Target: MAML3 (Mastermind-Like 3 (MAML3))

Andere Bezeichnung: MAML3 (MAML3 Produkte)

Synonyme: AV234550 antikoerper, BC049812 antikoerper, Mam-2 antikoerper, mKIAA1816 antikoerper, Glrp1 antikoerper, CAGH3 antikoerper, ERDA3 antikoerper, GDN antikoerper, MAM-2 antikoerper, MAM2 antikoerper, TNRC3 antikoerper, mastermind like 3 (Drosophila) antikoerper, mastermind-like transcriptional coactivator 3 antikoerper, mastermind like transcriptional coactivator 3 antikoerper, Maml3 antikoerper, MAML3 antikoerper

Hintergrund: Mastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.

Gen-ID: 55534