EpCAM Antikörper
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- Target Alle EpCAM (EPCAM) Antikörper anzeigen
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser EpCAM Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Keine Kreuzreaktivität
- Ratte (Rattus)
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- MCF-7 human breast cancer cells were used as the immunogen for this EpCAM antibody.
- Klon
- 323-A3
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product EPCAM Primärantikörper
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- Applikationshinweise
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1 mg/mL Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- Andere Bezeichnung
- EpCAM (EPCAM Produkte)
- Synonyme
- DIAR5 antikoerper, EGP-2 antikoerper, EGP314 antikoerper, EGP40 antikoerper, ESA antikoerper, HNPCC8 antikoerper, KS1/4 antikoerper, KSA antikoerper, M4S1 antikoerper, MIC18 antikoerper, MK-1 antikoerper, TACSTD1 antikoerper, TROP1 antikoerper, ECS-1 antikoerper, ECS1 antikoerper, ESA1 antikoerper, M17S1 antikoerper, EGP antikoerper, Ep-CAM antikoerper, sb:cb6 antikoerper, tacstd antikoerper, wu:fj17g02 antikoerper, zgc:110304 antikoerper, zgc:77119 antikoerper, tacstd1 antikoerper, MGC80540 antikoerper, EPCAM antikoerper, egp antikoerper, ksa antikoerper, m4s1 antikoerper, mk-1 antikoerper, cd326 antikoerper, egp40 antikoerper, mic18 antikoerper, trop1 antikoerper, ep-cam antikoerper, hegp-2 antikoerper, co17-1a antikoerper, ga733-2 antikoerper, CD326 antikoerper, Egp314 antikoerper, EpCAM1 antikoerper, GA733-2 antikoerper, Ly74 antikoerper, Tacsd1 antikoerper, Tacstd1 antikoerper, gp40 antikoerper, epithelial cell adhesion molecule antikoerper, flotillin 2 antikoerper, epithelial cell adhesion molecule S homeolog antikoerper, EPCAM antikoerper, FLOT2 antikoerper, epcam antikoerper, epcam.S antikoerper, Epcam antikoerper
- Hintergrund
- Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40 kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
- Gen-ID
- 4072
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