CDC20 Antikörper

Produktnummer ABIN3024578

Produktdetails anti-CDC20 Antikörper

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDC20 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G purified antibody

Immunogen: Denatured recombinant human protein was used as the immunogen for this Cdc20 antibody.

Klon: AR12

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cdc20 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cdc20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CDC20

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Andere Bezeichnung: Cdc20 (CDC20 Produkte)

Synonyme: X-FZY antikoerper, ba276h19.3 antikoerper, cdc20a antikoerper, fizzy1 antikoerper, p55cdc antikoerper, CDC20A antikoerper, bA276H19.3 antikoerper, p55CDC antikoerper, 2310042N09Rik antikoerper, C87100 antikoerper, cell division cycle 20 antikoerper, cell division cycle 20 L homeolog antikoerper, cell division cycle 20 homolog (S. cerevisiae) antikoerper, cdc20 antikoerper, CDC20 antikoerper, cdc20.L antikoerper, Cdc20 antikoerper

Hintergrund: Cyclins, regulatory subunits which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 kinase (p34Cdc2) exhibits kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.

Gen-ID: 991