CD8 alpha Antikörper

Produktnummer ABIN3024488

Produktdetails anti-CD8 alpha Antikörper

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD8 alpha Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: Human recombinant protein was used as the immunogen for this CD8a antibody.

Klon: C8-468

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD8a antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,Immunohistology (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CD8a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: CD8a (CD8A Produkte)

Synonyme: cd8 antikoerper, LOC100136450 antikoerper, BB154331 antikoerper, Ly-2 antikoerper, Ly-35 antikoerper, Ly-B antikoerper, Lyt-2 antikoerper, zgc:136643 antikoerper, CD8 antikoerper, Leu2 antikoerper, MAL antikoerper, p32 antikoerper, RHACD8-4 antikoerper, CD8a molecule antikoerper, uncharacterized LOC100125537 antikoerper, CD8 alpha antikoerper, CD8 antigen, alpha chain antikoerper, T-cell surface glycoprotein CD8 alpha chain antikoerper, Cd8a antikoerper, LOC100125537 antikoerper, LOC100136450 antikoerper, CD8A antikoerper, cd8a antikoerper, LOC100126556 antikoerper

Hintergrund: CD8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD8 and 4 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8a antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral Tcell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+/CD8-, and in T-lymphoblastic lymphoma/leukemia, they are often co-expressed. It is also found in littoral cell angioma of the spleen.

Gen-ID: 925

Pathways: T-Zell Rezeptor Signalweg