RAF1 Antikörper (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (AA 657-668)

Details for Product anti-RAF1 Antibody No. ABIN295906, Anbieter: Anmelden zum Anzeigen
Antigen
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • c-raf
  • craf
  • raf1
  • RAF1
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • raf
  • cRaf
  • Raf-1 proto-oncogene, serine/threonine kinase
  • Raf-1 proto-oncogene, serine/threonine kinase a
  • zinc fingers and homeoboxes 2
  • v-raf-leukemia viral oncogene 1
  • Raf-1 proto-oncogene, serine/threonine kinase S homeolog
  • RAF1
  • Raf1
  • raf1a
  • raf1
  • Zhx2
  • raf1.S
Epitop
AA 657-668
84
76
48
48
47
39
34
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34
31
31
22
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1
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1
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Reaktivität
Fledermaus, Rind (Kuh), Hund, Hamster, Pferd, Human, Affe, Maus, Kaninchen, Ratte (Rattus)
873
456
427
24
23
16
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8
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4
2
1
1
Wirt
Schaf
840
45
11
5
1
Klonalität
Polyklonal
Konjugat
Dieser RAF1 Antikörper ist unkonjugiert
45
45
44
29
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29
13
13
12
12
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12
12
12
12
12
12
1
1
1
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1
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1
Applikation
Immunoprecipitation (IP), Western Blotting (WB)
549
405
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108
61
35
30
18
15
10
2
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen 12 residue synthetic C terminal peptide (CTLTTSPRLPVF) corresponding to residues 637-648 of human Raf-1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).

Type of Immunogen: Synthetic peptide
Isotyp IgG
Spezifität Recognizes Raf-1, Mr ~70kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence homology.
Homologie Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).
Reinigung Protein A purified
Plasmids, Primers & others Plasmide, Primers & weitere RAF1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung RAF1 / RAF (RAF1 Antibody Abstract)
Hintergrund Name/Gene ID: RAF1
Subfamily: Raf
Family: Protein Kinase

Synonyms: RAF1, C-Raf, C-raf-1, CRAF, NS5, Raf-1, Proto-oncogene c-RAF, Oncogene RAF1, RAF, Raf1 kinase
Gen-ID 5894
UniProt P04049
Pathways MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Applikations-hinweise Approved: IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 μg/mL detects Raf-1 in RIPA lysates from 3T3/A31 cell lysates. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Raf-1 (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Included Positive Antigen Control: 3T3/A31 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. MAPK Activation and MBP Phosphorylation: 4 μg immunoprecipitates Raf-1. The immunocomplex was then used in a coupled phosphorylation assay to activate MEK1, which in turn activates MAP Kinase 2 that phosphorylates myelin basic protein in vitro.
Kommentare

Target Species of Antibody: Human

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeat freeze-thaw cycles.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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