RAF1 Antikörper (AA 657-668)
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- Target Alle RAF1 Antikörper anzeigen
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
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Bindungsspezifität
- AA 657-668
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Reaktivität
- Human, Maus, Ratte, Rind (Kuh), Affe, Kaninchen, Hund, Pferd, Fledermaus, Hamster
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Wirt
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Schaf
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Klonalität
- Polyklonal
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Konjugat
- Dieser RAF1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunoprecipitation (IP)
- Spezifität
- Recognizes Raf-1, Mr ~70kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence homology.
- Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).
- Aufreinigung
- Protein A purified
- Immunogen
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12 residue synthetic C terminal peptide (CTLTTSPRLPVF) corresponding to residues 637-648 of human Raf-1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
- Top Product
- Discover our top product RAF1 Primärantikörper
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- Applikationshinweise
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Approved: IP, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 μg/mL detects Raf-1 in RIPA lysates from 3T3/A31 cell lysates. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Raf-1 (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Included Positive Antigen Control: 3T3/A31 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. MAPK Activation and MBP Phosphorylation: 4 μg immunoprecipitates Raf-1. The immunocomplex was then used in a coupled phosphorylation assay to activate MEK1, which in turn activates MAP Kinase 2 that phosphorylates myelin basic protein in vitro. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
- Andere Bezeichnung
- RAF1 / RAF (RAF1 Produkte)
- Synonyme
- CRAF antikoerper, NS5 antikoerper, Raf-1 antikoerper, c-Raf antikoerper, c-raf antikoerper, craf antikoerper, raf1 antikoerper, RAF1 antikoerper, 6430402F14Rik antikoerper, AA990557 antikoerper, BB129353 antikoerper, Craf1 antikoerper, D830050J10Rik antikoerper, v-Raf antikoerper, raf antikoerper, cRaf antikoerper, Raf-1 proto-oncogene, serine/threonine kinase antikoerper, Raf-1 proto-oncogene, serine/threonine kinase a antikoerper, v-raf-leukemia viral oncogene 1 antikoerper, Raf-1 proto-oncogene, serine/threonine kinase S homeolog antikoerper, RAF1 antikoerper, Raf1 antikoerper, raf1a antikoerper, raf1 antikoerper, raf1.S antikoerper
- Hintergrund
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Name/Gene ID: RAF1
Subfamily: Raf
Family: Protein Kinase
Synonyms: RAF1, C-Raf, C-raf-1, CRAF, NS5, Raf-1, Proto-oncogene c-RAF, Oncogene RAF1, RAF, Raf1 kinase - Gen-ID
- 5894
- UniProt
- P04049
- Pathways
- MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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