Macrophage Surface Antigen Antikörper

Produktnummer ABIN289946

Produktdetails

Target: Macrophage Surface Antigen

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (IHC)

Aufreinigung: The splenic lymphocytes from BALB/c mouse, immunized with human alveolarmacrophages, were fused to myeloma NS-1 cells. The screening of thehybridoma cells was performed on cryostat sections of human lung. The cellline (AM-3K) with positive reaction was grown in ascitic fluid of BALB/cmouse, from which the antibody was purified by Protein G affinity chromatography.

Immunogen: human alveolar macrophages

Klon: AM-3K

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Working dilution for immunohistochemistry: 10ug/ml, on frozen sections and paraffin sections. Antigen retrieval (microwave 10min, 0.01M citrate buffer, pH2.0) recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.25 mg/ml

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Konservierungsmittel: ProClin

Lagerung: -20 °C

Antigendetails

Target: Macrophage Surface Antigen

Hintergrund: This anti-macrophage monoclonal antibody, AM-3K, was produced by using human alveolarmacrophages as immunogen. AM-3K reacts intensely with most of macrophages in lymphoreticular organsand in many other organs and tissues. AM-3K also reacts with the macrophages in many pathologicalconditions. However, this antibody does not react with dendritic cell population, such as epidermal Langerhans cells, interdigitating cells in the paracortex of lymph nodes, nor follicular dendritic cells.Lymphocytes, granulocytes and freshly isolated blood monocytes are also negative. Reaction products for AM-3K were found on the cytoplasmic membrane of macrophages by immunoelectron microscopy. In both cryostat sections and formalin-fixed paraffin sections, this antibody recognizes the antigenpresenting on the cell surface membrane of tissue macrophages, but not monocytes or dendritic cells. The molecular weights of the antigen recognized by AM-3K are 120 and 70 kDa.