VEROTOXIN (Subunit A) Antikörper

Produktnummer ABIN2859493

Produktdetails

Target: VEROTOXIN

Bindungsspezifität: Subunit A

Reaktivität: E. coli

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Enzyme Immunoassay (EIA), Neutralization (Neut)

Aufreinigung: Protein A affinity chromatography

Reinheit: > 90 % pure

Klon: 557

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: ELISA. Neutralization.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL ( OD280nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2, containing 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, aliquot and store at -20 °C.

Antigendetails

Target: VEROTOXIN

Hintergrund: Verotoxins are produced by certain enterohaemorrhagic strains of Escherichia coli. Serotype O157:H7 has been the strain most commonly associated with large food bourne outbreaks and has been implicated in a wide spectrum of diseases including blood diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome. The main sources of infection are contaminated foods of animal origin such as meat and dairy products. Verotoxins, also called Shiga like toxins( SLT) because of their similarity to toxins produced by Shigella dysenteriae, are classified into two main categories: verotoxin 1 (VT1, SLT1, Stx1) and 2 (VT2, SLT2, Stx2). Verotoxin production is the most common criteria for the detection of this group of bacteria rather than tests for the cytopathic effect on Vero cells that gave the toxins their name. Verotoxins contain a single A subunit and multiple copies of a B subunit. The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits.Synonyms: SLT-2 A subunit, SLT-2a, SLT-IIa, Verocytotoxin 2 subunit A, Verotoxin 2 subunit A, rRNA N-glycosidase 2, stx2A, stxA2

Gen-ID: 10730

UniProt: P09385