Proteasome 20S alpha 1 (Center) Antikörper
-
- Target
- Proteasome 20S alpha 1
- Bindungsspezifität
- Center
- Reaktivität
- Human, Maus, Ratte
- Wirt
- Kaninchen
- Klonalität
- Polyklonal
- Applikation
- Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- Kreuzreaktivität
- Rhesusaffen, Rind (Kuh)
- Kreuzreaktivität (Details)
- Rhesus Monkey (100 %), Bovine (100 %)
- Produktmerkmale
-
Rabbit Polyclonal antibody to Proteasome 20S alpha 1 (proteasome (prosome, macropain) subunit, alpha type, 1)
Proteasome 20S alpha 1 antibody [N1C3] - Aufreinigung
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 1. The exact sequence is proprietary.
- Isotyp
- IgG
-
- Applikationshinweise
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- Kommentare
-
Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Konservierungsmittel
- Thimerosal (Merthiolate)
- Vorsichtsmaßnahmen
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
- Target
- Proteasome 20S alpha 1
- Hintergrund
-
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Cellular Localization: Cytoplasm , Nucleus - Molekulargewicht
- 30 kDa
- Gen-ID
- 5682
-