GRP78 Antikörper (Internal Region)
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- Target Alle GRP78 (HSPA5) Antikörper anzeigen
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human, Ratte, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser GRP78 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Kreuzreaktivität
- Huhn, Rind (Kuh), Rhesusaffen
- Kreuzreaktivität (Details)
- Mouse (100 %), Rat (100 %), Chicken (100 %), Rhesus Monkey (100 %), Bovine (100 %)
- Produktmerkmale
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Rabbit polyclonal antibody to Grp78 (heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa))
Grp78 antibody [N2C1], Internal - Aufreinigung
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.
- Isotyp
- IgG
- Top Product
- Discover our top product HSPA5 Primärantikörper
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- Applikationshinweise
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*
- Kommentare
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Positive Control: 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.72 mg/mL
- Buffer
- 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Konservierungsmittel
- Thimerosal (Merthiolate)
- Vorsichtsmaßnahmen
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Metformin attenuates the D‑galactose‑induced aging process via the UPR through the AMPK/ERK1/2 signaling pathways." in: International journal of molecular medicine, Vol. 45, Issue 3, pp. 715-730, (2020) (PubMed).
: "The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways." in: Redox biology, Vol. 12, pp. 987-1003, (2017) (PubMed).
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Metformin attenuates the D‑galactose‑induced aging process via the UPR through the AMPK/ERK1/2 signaling pathways." in: International journal of molecular medicine, Vol. 45, Issue 3, pp. 715-730, (2020) (PubMed).
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- Target
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
- Andere Bezeichnung
- Grp78 (HSPA5 Produkte)
- Synonyme
- GRP78 antikoerper, BiP antikoerper, GRP-78 antikoerper, grp78 antikoerper, hspa5a antikoerper, BIP antikoerper, MIF2 antikoerper, AL022860 antikoerper, AU019543 antikoerper, Bip antikoerper, D2Wsu141e antikoerper, D2Wsu17e antikoerper, Grp78 antikoerper, Hsce70 antikoerper, SEZ-7 antikoerper, Sez7 antikoerper, baffled antikoerper, mBiP antikoerper, cb865 antikoerper, fb60h09 antikoerper, fi36d04 antikoerper, wu:fb60h09 antikoerper, wu:fi36d04 antikoerper, zgc:55994 antikoerper, zgc:77606 antikoerper, 78 kDa glucose-regulated protein antikoerper, heat shock protein family A (Hsp70) member 5 antikoerper, BiP/GRP78 antikoerper, glucose-regulated protein 78 antikoerper, putative glucose-regulated protein 78 antikoerper, Hsp70 family ATPase KAR2 antikoerper, heat shock protein family A (Hsp70) member 5 S homeolog antikoerper, heat shock 70 kDa protein 5a antikoerper, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) antikoerper, heat shock protein 5 antikoerper, heat shock protein family A member 5 antikoerper, CpipJ_CPIJ003550 antikoerper, HSPA5 antikoerper, grp78 antikoerper, LOC100533358 antikoerper, BiP/grp78 antikoerper, Tc00.1047053506585.40 antikoerper, Tb11.02.5450 antikoerper, Tb11.02.5500 antikoerper, LMJF_28_1200 antikoerper, KAR2 antikoerper, LOC100135840 antikoerper, hspa5.S antikoerper, hspa5 antikoerper, Hspa5 antikoerper
- Hintergrund
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When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
Cellular Localization: Endoplasmic reticulum lumen , Melanosome - Molekulargewicht
- 72 kDa
- Gen-ID
- 3309
- Pathways
- Thyroid Hormone Synthesis, ER-Nucleus Signaling
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