POLR3A Antikörper (Middle Region)

Produktnummer ABIN2783481

Produktdetails anti-POLR3A Antikörper

Target: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Rind (Kuh), Pferd, Meerschweinchen, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: AYFGQKDSVC GVSECIIMGI PMNIGTGLFK LLHKADRDPN PPKRPLIFDT

Homologie: Cow: 79%, Dog: 86%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 86%, Yeast: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against POLR3A. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLR3A

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 1390 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu POLR3A

Target: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Andere Bezeichnung: POLR3A (POLR3A Produkte)

Synonyme: 9330175N20Rik antikoerper, BC053071 antikoerper, RPC1 antikoerper, RPC155 antikoerper, RGD1305574 antikoerper, ADDH antikoerper, HLD7 antikoerper, hRPC155 antikoerper, polymerase (RNA) III (DNA directed) polypeptide A antikoerper, RNA polymerase III subunit A antikoerper, Polr3a antikoerper, POLR3A antikoerper

Hintergrund: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
Alias Symbols: RPC1, RPC155, hRPC155, ADDH, HLD7
Protein Interaction Partner: SUMO2, UBC, ASB13, NEDD4, POLR3H, VPS18, POLR3B, POLR1D, CRCP, POLR1A, POLR3C, POLR1C, SMARCA5, POLR2H, POLR2E, POLR3D, IKBKG, PCBD1, EWSR1, RPAP2, RPAP3, RPAP1, GPN1, RAE1, POLR3E, PKP2, GTF2B,
Protein Size: 1390

Molekulargewicht: 156 kDa

Gen-ID: 11128

NCBI Accession: NM_007055, NP_008986

UniProt: O14802