TRIM25 Antikörper

Produktnummer ABIN272231

Produktdetails anti-TRIM25 Antikörper

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRIM25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of EFP protein. (region surrounding Asp222)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Andere Bezeichnung: TRIM25 / RNF147 (TRIM25 Produkte)

Synonyme: zgc:55773 antikoerper, MGC130751 antikoerper, EFP antikoerper, RNF147 antikoerper, Z147 antikoerper, ZNF147 antikoerper, AA960166 antikoerper, AL022677 antikoerper, Zfp147 antikoerper, Efp antikoerper, Znf147 antikoerper, E3 ubiquitin/ISG15 ligase TRIM25 antikoerper, tripartite motif containing 25 antikoerper, E3 ubiquitin/ISG15 ligase TRIM25-like L homeolog antikoerper, microRNA 3614 antikoerper, tripartite motif-containing 25 antikoerper, LOC100146440 antikoerper, trim25 antikoerper, LOC100490741.L antikoerper, TRIM25 antikoerper, MIR3614 antikoerper, Trim25 antikoerper

Hintergrund: EFP (estrogen-responsive finger protein) is a transcription factor, the content of which is regulated by estrogen. It has been identified as a member of the RING finger family, a family of proteins containing a Zn2+ binding domain designated the C3HC4 or RING finger. EFP also contains two B box domains and a coiled-coil region (a transactivation domain), which are characteristic of a subgroup of the RING finger family. Estrogen regulates the growth, differentiation and function of target cells in a variety of tissues, however, few genes have been shown to be directly regulated by estrogen. It has been speculated that EFP may mediate estrogen activity in a signaling cascade in which estrogen-ER binding to the estrogen responsive element (ERE) downstream of the EFP gene upregulates EFP gene expression. The EFP gene product may then activate transcription of secondary estrogen responsive genes. Additional studies indicate that the EFP promoter may be regulated by multiple elements and their interacting factors.Synonyms: EFP, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, ZNF147, Zinc finger protein 147

Molekulargewicht: approx. 71 kDa

Gen-ID: 7706

NCBI Accession: NP_005073

UniProt: Q14258