RAN Antikörper

Produktnummer ABIN271799

Produktdetails anti-RAN Antikörper

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: RAN antibody detects endogenous levels of RAN protein. (region surrounding E202)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: PBS, pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu RAN

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Andere Bezeichnung: RAN (RAN Produkte)

Synonyme: ARA24 antikoerper, Gsp1 antikoerper, TC4 antikoerper, ran antikoerper, ara24 antikoerper, gsp1 antikoerper, ran-1 antikoerper, tc4 antikoerper, RAN antikoerper, RANP1 antikoerper, AAF30287 antikoerper, CG1404 antikoerper, Dmel\CG1404 antikoerper, Ran antikoerper, dran antikoerper, l(1)G0075 antikoerper, ran10A antikoerper, fc16b04 antikoerper, wu:fc16b04 antikoerper, RAN, member RAS oncogene family antikoerper, RAN, member RAS oncogene family S homeolog antikoerper, CG1404 gene product from transcript CG1404-RC antikoerper, RAN antikoerper, Ran antikoerper, ran.S antikoerper, ran antikoerper

Hintergrund: The small Ras-related protein Ran, also called TC4, is a 24 kDa, nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). The abundant GEF, RCC1 (regulator of chromosome condensation 1), increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A 23 kDa protein designated Ran BP1 has no intrinsic GAP activity, and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. The 358 kDa protein Ran BP2 has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.Synonyms: ARA24, Androgen receptor-associated protein 24, GTP-binding nuclear protein Ran, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein

Molekulargewicht: approx. 25 kDa

Gen-ID: 5901

NCBI Accession: NP_006316

UniProt: P62826

Pathways: Regulatorische RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus