NKG2A/C/E Antikörper

Produktnummer ABIN2689860

Produktdetails anti-NKG2A/C/E Antikörper

Target: NKG2A/C/E (KLRC1/2/3) (Killer Cell Lectin-Like Receptor Subfamily C, Member 1/2/3 (KLRC1/2/3))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser NKG2A/C/E Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Blocking Reagent (BR)

Marke: BD Pharmingen™

Produktmerkmale: The 20d5 antibody reacts with NKG2A, C, and E on a subset of NK and NK-T cells in most strains tested (eg, AKR/J, BALB/c, C3H/He, C57BL/6, CBA/J, DBA/1, FVB/N, 129/Sv, NOD, SWR, and most DBA/2 substrains, but not DBA/2J). The NKG2 Molecules are a family of lectin-like receptors that form heterodimers with CD94 on the surface of NK cells. DBA/2J mice do not express CD94, and the lack of CD94 is responsible for the absence of NKG2 expression in this substrain. NKG2 receptors are also expressed on CD8+ T lymphocytes activated in vivo and in vitro. The heterodimers of CD94 with NKG2A, C, or E recognize Qa-1, a nonclassical MHC class I antigen, presenting the Qdm peptide. Studies of CD94/NKG2 heterodimers on human NK cells have demonstrated that the NKG2 components mediate signal transduction for the receptor, with NKG2A being inhibitory and NKG2C being stimulatory. The CD94/NKG2E heterodimer is also thought to be stimulatory. The mouse NKG2A molecule contains two intracytoplasmic sequences that resemble the ITIM (Immunoreceptor Tyrosine- based Inhibitory Motif) concensus sequence. NKG2A transcripts have been shown to be up to 20-fold more abundant than NKG2C and NKG2E mRNA in NK cells of adult mice. The CD94/NKG2 receptors show increased expression on neonatal NK cells compared to the Ly-49 MHC class I receptors, suggesting that CD94/NKG2 receptors and their ligand, Qa-1, may play a role in maintenance of self-tolerance in developing NK cells. The 20d5 antibody is useful for identification of NK cells expressing functional CD94/NKG2 receptors, in contrast to the non-functional CD94 expressed alone, and it blocks the binding of Qdm-complexed Qa-1b tetramers to CD94/NKG2-transfected CHO cells. Expression of NKG2A/C/E on mouse splenic NK cells. C57BL/6 splenocytes were simultaneously stained with PE-conjugated anti-mouse NK-1.1 mAb PK136 (Cat. no. 557391) and purified mAb 20d5 (Right panel), followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 (Cat. no. 553896). The NK1.1+NKG2+ and NK1.1+NKG2- subsets are detected. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse NKG2A/C/E - Purified - Clone 20d5 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Transfected cell line

Klon: 20d5

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-NKG2A/C/E Antikörper (ABIN2689860)

McMahon, Zajac, Jamieson, Corral, Hammer, Ahmed, Raulet: "Viral and bacterial infections induce expression of multiple NK cell receptors in responding CD8(+) T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 3, pp. 1444-52, (2002) (PubMed).

Vance, Jamieson, Cado, Raulet: "Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 868-73, (2002) (PubMed).

Kubota, Kubota, Lohwasser, Mager, Takei: "Diversity of NK cell receptor repertoire in adult and neonatal mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 212-6, (1999) (PubMed).

Lohwasser, Hande, Mager, Takei: "Cloning of murine NKG2A, B and C: second family of C-type lectin receptors on murine NK cells." in: European journal of immunology, Vol. 29, Issue 3, pp. 755-61, (1999) (PubMed).

Yokoyama: "Natural killer cell receptors." in: Current opinion in immunology, Vol. 10, Issue 3, pp. 298-305, (1998) (PubMed).

Details zu NKG2A/C/E

Target: NKG2A/C/E (KLRC1/2/3) (Killer Cell Lectin-Like Receptor Subfamily C, Member 1/2/3 (KLRC1/2/3))

Andere Bezeichnung: NKG2A/C/E (KLRC1/2/3 Produkte)