O-GlcNAc Antikörper
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- Target
- O-GlcNAc
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Reaktivität
- Chemical
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Unkonjugiert
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Applikation
- Western Blotting (WB), Immunoprecipitation (IP)
- Aufreinigung
- IgM purification
- Immunogen
- This antibody was raised against a peptide containing O-GlcNAc - serine. This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and threonine.
- Klon
- CTD110-6
- Isotyp
- IgM
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 μg/μL
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Antibodies in solution can be stored at -20 °C for 2 years.
- Haltbarkeit
- 6 months
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-
Glycopeptide-specific monoclonal antibodies suggest new roles for O-GlcNAc." in: Nature chemical biology, Vol. 6, Issue 5, pp. 338-43, (2010) (PubMed).
: "Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine." in: Analytical biochemistry, Vol. 293, Issue 2, pp. 169-77, (2001) (PubMed).
: "
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Glycopeptide-specific monoclonal antibodies suggest new roles for O-GlcNAc." in: Nature chemical biology, Vol. 6, Issue 5, pp. 338-43, (2010) (PubMed).
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- Target
- O-GlcNAc
- Substanzklasse
- Viral Protein
- Hintergrund
- O-GlcNAc (b-O-linked N-acetylglucosamine) is a ubiquitous and dynamic post-translational modification present on numerous cytoplasmic and nuclear proteins and is thought to play a regulatory role in numerous cellular processes including homeostatic mechanisms and gene expression. OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residues whereas OGA / O-GlcNAcase (O- linked N-acetylglucosamine (GlcNAc)-ase) is a glycosidase that removes O-GlcNAc. O-GlcNAc modifications have been described to directly regulate the activities of a variety of transcription factors including Sp1, estrogen receptors, STAT5, NF-κB, p53, YY1, Elf-1, c-Myc, Rb, PDX-1, CREB, fork-head, and others. In addition, RNA polymerase II and the basal transcription factors are also extensively modified by O-GlcNAc. O-GlcNAc modification has been reported to suppress or enhance transcription, depending on the promoter and other associated proteins.
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