IGJ Antikörper (Biotin)

Produktnummer ABIN2624384

Produktdetails anti-IGJ Antikörper (Biotin)

Target: IGJ (Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IGJ Antikörper ist konjugiert mit Biotin

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Dot Blot (DB)

Spezifität: Human J chain of dimeric IgA.

Aufreinigung: Purified IgG

Immunogen: Human J chain is a polypeptide folded within the structure of the polymeric immunoglobulin, resulting in a very limited exposure of J chain antigenic determinants. The antiserum is raised against the isolated and purified J chain. J chains isolated from polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity. Human J chain is distinct from all other chain components of polymeric IgA and IgM. It has a unique primary structure as shown by sequence analyses. Fr

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: DB, ELISA, ICC, IHC, IHC-P

Usage: In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), this immunoconjugate shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA, secretory IgA and IgM. A reaction of complete identity is obtained with precipitated highly purified J chain and with the single of precipitation obtained with normal human serum after two hours incubation with 9 M urea and 0.2 M mercaptoethanol at  pH 8.6. In a competitive radioimmunoassay no inhibition is obtained with monomeric IgA, polyclonal IgG, reduced and alkylated alpha, mu, gamma, kappa and lambda light chains (less than 1 percent over background). In immunocytochemical and immunohistochemical techniques for the detection of human J chain at the cellular and subcellular level in appropriately treated cell and tissue substrates. as detection reagent in nonisotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:200 and 1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu IGJ

Target: IGJ (Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))

Andere Bezeichnung: JCHAIN / Ig J Chain (IGJ Produkte)

Synonyme: IGJ antikoerper, DKFZp469K0627 antikoerper, IGCJ antikoerper, JCH antikoerper, 9530090F24Rik antikoerper, AI323815 antikoerper, Jch antikoerper, joining chain of multimeric IgA and IgM antikoerper, immunoglobulin joining chain antikoerper, Jchain antikoerper, JCHAIN antikoerper

Hintergrund: Name/Gene ID: JCHAIN

Synonyms: JCHAIN, IGCJ, Immunoglobulin J chain, IGJ, IgJ chain, JCH

Gen-ID: 3512