CBR antikoerper, SDR21C1 antikoerper, hCBR1 antikoerper, OPLL antikoerper, AI747156 antikoerper, Col6a-1 antikoerper, RGD1565398 antikoerper, COL6A1 antikoerper, carbonyl reductase 1 antikoerper, collagen type VI alpha 1 chain antikoerper, collagen, type VI, alpha 1 antikoerper, collagen, type VI, alpha 1 L homeolog antikoerper, collagen type VI alpha 3 chain antikoerper, collagen alpha-1(VI) chain antikoerper, CBR1 antikoerper, COL6A1 antikoerper, Col6a1 antikoerper, col6a1.L antikoerper, COL6A3 antikoerper, col6a1 antikoerper, LOC100623720 antikoerper
Hintergrund
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.