Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Aufreinigung
GABRA4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GABRA4 antibody was raised against a 13 amino acid synthetic peptide near the internal region of GABRA4.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 70 kDa band observed in human brain (cerebellum and frontal cortex) lysates (calculated MW of 61.6 kDa according to NP_000800.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human cortex shows patchy staining near or at the neuronal cell membrane. Recommended concentration, 2-4 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
GABRA4
(gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))