VTCN1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
VTCN1 antibody was raised against a 12 amino acid synthetic peptide near the internal region of VTCN1.
VTCN1
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
RB25449
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:64,000. Approximately 60 kDa band observed in human lung, spleen and thymus lysates (calculated MW of 30.9 kDa according to NP_078902.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Simon et al, tumor Res. 2006 Feb 1,66 (3) :1570-5., PMID: 16452214). In transfected HEK293 transiently expressing B7-H4 the band of approximately 60 kDa is also observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
VTCN1
(V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))