Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Spezifität
This antibody is expected to recognize all 5 isoforms of SCARF1. Epitope corresponding to aa 20-31, mapping to the mature human SCARF1 / SREC1 protein.
Aufreinigung
SREC antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SREC antibody was raised against an 11 amino acid synthetic peptide near the internal region of SREC.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 85-90 kDa band observed in human heart lysates (calculated MW of 87.4 kDa according to NP_003684,). Recommended concentration: 0.3-1.0 μg/mL. an additional faint band of 28 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western Blot.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.