RAB11FIP1 Antikörper (Internal Region)

Produktnummer ABIN2466172

Produktdetails anti-RAB11FIP1 Antikörper

Target: RAB11FIP1 (Rab11 Family-Interacting Protein 1 (RAB11FIP1))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser RAB11FIP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: This antibody is expected to recognize isoform 1 (NP_079427.3) and isoform 3 (NP_001002814.1).

Aufreinigung: RAB11FIP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: RAB11FIP1 antibody was raised against a 13 amino acid synthetic peptide near the internal region of RAB11FIP1.

Anwendungsinformationen

Applikationshinweise: ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 110 kDa, 50 kDa and 38 kDa in human breast lysate after 1 μg/mL antibody staining.0 kDa according to NP_079427.3, and 137 kDa according to NP_001002814.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Please note that an additional 55 kDa was only partly blocked by the immunizing peptide, this band may not be specific. Approximately 80 kDa band observed in HeLa lysate (calculated MW of 71.0 kDa according to NP_079427.3). The observed molecular weight of 80 kDa corresponds to earlier findings in literature with different antibodies (Lindsay et al, J Biol Chem. 2002 Apr 5,277(14):12190-9. PMID 11786538.). Recommended concentration: 0.1-0.3 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20 °C.

Details zu RAB11FIP1

Target: RAB11FIP1 (Rab11 Family-Interacting Protein 1 (RAB11FIP1))

Andere Bezeichnung: RAB11FIP1 (RAB11FIP1 Produkte)

Synonyme: zgc:122989 antikoerper, NOEL1A antikoerper, RCP antikoerper, rab11-FIP1 antikoerper, 2010200K21Rik antikoerper, 4833414G05Rik antikoerper, Rcp antikoerper, RGD1562965 antikoerper, Rab11-FIP1 antikoerper, RAB11 family interacting protein 1 antikoerper, RAB11 family interacting protein 1 (class I) a antikoerper, RAB11 family interacting protein 1 (class I) antikoerper, RAB11FIP1 antikoerper, rab11fip1a antikoerper, Rab11fip1 antikoerper

Gen-ID: 80223

NCBI Accession: NP_079427, NP_001002814

UniProt: Q6WKZ4