Proenkephalin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Proenkephalin antibody was raised against a 12 amino acid synthetic peptide near the internal region of Proenkephalin.
PENK
Reaktivität: Human
WB
Wirt: Maus
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 37 kDa band observed in human adrenal gland lysates (calculated MW of 30.8 kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrinology. 1998 Apr,139 (4) :2137-45, PMID: 9529003). Recommended concentration: 1-3 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.