This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Aufreinigung
NET1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
NET1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of NET1.
NET1
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 85 kDa in human placenta and a very strong band at approximately 40-50 kDa in human skeletal muscle lysates after 0.1 μg/mL staining.7 kDa according to NP_001040625.1 and of 61.9 kDa according to NP_005854.2. Immunohistochemistry: In paraffin embedded human tonsil shows staining of fibrous structures. Recommended concentration, 2.5 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.