This antibody is expected to recognize both reported isoforms (NP_057412.1 and NP_005676.2).
Aufreinigung
GTF2IRD1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GTF2IRD1 antibody was raised against a 14 amino acid synthetic peptide near the internal region of GTF2IRD1.
GTF2IRD1
Reaktivität: Human
ELISA, IHC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 30 kDa and 40 kDa in human heart lysate after 0.3 μg/mL antibody staining. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
GTF2IRD1
(General Transcription Factor II I Repeat Domain-Containing 1 (GTF2IRD1))