Pyruvate Dehydrogenase (C-Term) Antikörper
Reaktivität: Human
WB, IHC, ELISA
Wirt: Ziege
Polyclonal
unconjugated
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- Target
- Pyruvate Dehydrogenase
- Bindungsspezifität
- C-Term
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Spezifität
- This antibody is expected to recognise all reported isoforms.
- Aufreinigung
- Pyruvate Dehydrogenase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Immunogen
- Pyruvate Dehydrogenase antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of Pyruvate Dehydrogenase.
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- Applikationshinweise
- ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 50 kDa band observed in human pancreas lysate (calculated MW of 54.2 kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approximately 50 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows cytoplasmic staining of acinar cells, islets of Langerhans and peri-pancreatic fat cells. Recommended concentration, 1-3 μg/mL.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 500 μg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20 °C.
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