Amphiphysin Antikörper (Internal Region)

Produktnummer ABIN2465168

Produktdetails anti-Amphiphysin Antikörper

Target: Amphiphysin (AMPH)

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Amphiphysin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Spezifität: This antibody is expected to recognize both reported isoforms (NP_001626.1 and NP_647477.1).

Aufreinigung: Amphiphysin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Amphiphysin antibody was raised against a 12 amino acid synthetic peptide near the internal region of Amphiphysin.

Anwendungsinformationen

Applikationshinweise: ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 125 kDa band observed in mouse brain lysates (calculated MW of 76.3 kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct,8 (10) :2003-15., PMID: 9348539). Recommended concentration: 0.1-0.5 μg/mL. A minor band of unknown identity was also consistently observed at 45 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20 °C.

Details zu Amphiphysin

Target: Amphiphysin (AMPH)

Andere Bezeichnung: Amphiphysin (AMPH Produkte)

Synonyme: Amp antikoerper, CG8604 antikoerper, DAMP antikoerper, Damp antikoerper, Dmel\\CG8604 antikoerper, amph antikoerper, dAmph antikoerper, damph antikoerper, AMPH antikoerper, Amph antikoerper, GB16263 antikoerper, AMPH1 antikoerper, Amph1 antikoerper, wu:fq25h04 antikoerper, zgc:73193 antikoerper, amphiphysin antikoerper, Amphiphysin antikoerper, amphiphysin L homeolog antikoerper, AMPH antikoerper, Amph antikoerper, amph antikoerper, LOC409851 antikoerper, amph.L antikoerper, LOC100343468 antikoerper

Gen-ID: 273

NCBI Accession: NP_001626, NP_647477

UniProt: P49418