RBM20 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
RBM20 antibody was raised against an 11 amino acid synthetic peptide near the internal region of RBM20 (aa 717-727).
Approximately 150 kDa band observed in mouse heart lysates (calculated MW of 130 kDa according to mouse NP_001164318.1). Recommended concentration: 1-3 μg/mL. an additional band of 25 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western Blot.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.