POU2F1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
POU2F1 antibody was raised against a 15 amino acid synthetic peptide near the internal region (near N-Terminus) of POU2F1 (near N-Terminus).
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 100 kDa band observed in nuclear HeLa lysate (calculated MW of 76.5 kDa according to NP_002688.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
POU2F1
(POU Domain, Class 2, Transcription Factor 1 (POU2F1))