ITIH4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ITIH4 antibody was raised against a 15 amino acid synthetic peptide near the internal region of ITIH4.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 150 kDa band in human liver and lung lysates after 0.03 μg/mL antibody staining. The 150 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 4-6 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.