RAD18 Antikörper

Produktnummer ABIN2462678

Produktdetails anti-RAD18 Antikörper

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RAD18.

Anwendungsinformationen

Applikationshinweise: RAD18 antibody can be used for detection of RAD18 by ELISA at 1:312500. RAD18 antibody can be used for detection of RAD18 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RAD18 antibody at -20 °C.

Details zu RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Andere Bezeichnung: RAD18 (RAD18 Produkte)

Synonyme: RNF73 antikoerper, 2810024C04Rik antikoerper, Rad18sc antikoerper, zgc:55577 antikoerper, RAD18 antikoerper, DDBDRAFT_0218120 antikoerper, DDBDRAFT_0304878 antikoerper, DDB_0218120 antikoerper, DDB_0304878 antikoerper, DKFZp469H0810 antikoerper, RAD18, E3 ubiquitin protein ligase antikoerper, RAD18 E3 ubiquitin protein ligase antikoerper, E3 ubiquitin-protein ligase RAD18 antikoerper, E3 ubiquitin-protein ligase antikoerper, DNA repair protein and ATPase antikoerper, C3HC4-type zinc finger-containing protein antikoerper, RAD18 antikoerper, Rad18 antikoerper, rad18 antikoerper

Hintergrund: RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18 is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molekulargewicht: 56 kDa

Gen-ID: 56852

NCBI Accession: NP_064550

UniProt: Q9NS91