SF3A1 Antikörper

Produktnummer ABIN2462195

Produktdetails anti-SF3A1 Antikörper

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SF3A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3A1.

Anwendungsinformationen

Applikationshinweise: SF3A1 antibody can be used for detection of SF3A1 by ELISA at 1:62500. SF3A1 antibody can be used for detection of SF3A1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SF3A1 antibody at -20 °C.

Details zu SF3A1

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Andere Bezeichnung: SF3A1 (SF3A1 Produkte)

Synonyme: Dmel\\CG16941 antikoerper, SF3a1 antikoerper, SF3a120 antikoerper, DDBDRAFT_0168938 antikoerper, DDBDRAFT_0233180 antikoerper, DDB_0168938 antikoerper, DDB_0233180 antikoerper, 1200014H24Rik antikoerper, 5930416L09Rik antikoerper, AI159724 antikoerper, si:dz150f13.2 antikoerper, wu:fc38e01 antikoerper, wu:fc50a11 antikoerper, wu:fj37c05 antikoerper, zgc:65786 antikoerper, PRP21 antikoerper, PRPF21 antikoerper, SAP114 antikoerper, SF3A120 antikoerper, CG16941 gene product from transcript CG16941-RA antikoerper, SWAP/Surp domain-containing protein antikoerper, splicing factor 3a, subunit 1 antikoerper, splicing factor 3a subunit 1 antikoerper, CG16941 antikoerper, sf3a1 antikoerper, spl1 antikoerper, Sf3a1 antikoerper, SF3A1 antikoerper

Hintergrund: SF3A1 is the subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs.This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molekulargewicht: 89 kDa

Gen-ID: 10291

NCBI Accession: NP_005868

UniProt: Q15459

Pathways: Ribonucleoprotein Complex Subunit Organization