Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Aufreinigung
Antibody is purified by protein A chromatography method.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CIon Channel.
Applikationshinweise
CIon Channel antibody can be used for detection of CIon Channel by ELISA at 1:62500. CIon Channel antibody can be used for detection of CIon Channel by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Konzentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handhabung
As with any antibody avoid repeat freeze-thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
For short periods of storage (days) store at 4 °C. For longer periods of storage, store CIon Channel antibody at -20 °C.
Target
CIon Channel
Hintergrund
CIon Channel represents a new subfamily of the HMG-box superfamily. Expression of the Cic gene is predominantly restricted to immature granule cells in the cerebellum, hippocampus and olfactory bulb in the CNS