ADD2 Antikörper

Produktnummer ABIN2459527

Produktdetails anti-ADD2 Antikörper

Target: ADD2 (Adducin 2 (Beta) (ADD2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ADD2.

Anwendungsinformationen

Applikationshinweise: ADD2 antibody can be used for detection of ADD2 by ELISA at 1:312500. ADD2 antibody can be used for detection of ADD2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ADD2 antibody at -20 °C.

Details zu ADD2

Target: ADD2 (Adducin 2 (Beta) (ADD2))

Andere Bezeichnung: ADD2 (ADD2 Produkte)

Synonyme: si:dkey-202n14.5 antikoerper, ADDB antikoerper, 2900072M03Rik antikoerper, add97 antikoerper, beta-ADD antikoerper, adducin 2 antikoerper, adducin 2 (beta) antikoerper, ADD2 antikoerper, add2 antikoerper, Add2 antikoerper

Hintergrund: Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.

Molekulargewicht: 80 kDa

Gen-ID: 119

NCBI Accession: NP_001608

UniProt: P35612

Pathways: Regulation of Actin Filament Polymerization