CDC45 Antikörper

Produktnummer ABIN2459485

Produktdetails anti-CDC45 Antikörper

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC45 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDC45L.

Anwendungsinformationen

Applikationshinweise: CDC45L antibody can be used for detection of CDC45L by ELISA at 1:12500. CDC45L antibody can be used for detection of CDC45L by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CDC45L antibody at -20 °C.

Details zu CDC45

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Andere Bezeichnung: CDC45L (CDC45 Produkte)

Synonyme: cdc45l antikoerper, cdc45l2 antikoerper, porc-pi-1 antikoerper, CDC45L antikoerper, CDC45L2 antikoerper, PORC-PI-1 antikoerper, Cdc45l antikoerper, cell division cycle 45 antikoerper, cell division cycle 45 S homeolog antikoerper, cell division cycle 45 L homeolog antikoerper, cdc45 antikoerper, CDC45 antikoerper, cdc45.S antikoerper, Cdc45 antikoerper, cdc45.L antikoerper

Hintergrund: CDC45L was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molekulargewicht: 65 kDa

Gen-ID: 8318

NCBI Accession: NP_003495

UniProt: O75419

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA