NEK3 Antikörper

Produktnummer ABIN2459259

Produktdetails anti-NEK3 Antikörper

Target: NEK3 (NIMA related kinase 3 (NEK3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NEK3.

Anwendungsinformationen

Applikationshinweise: NEK3 antibody can be used for detection of NEK3 by ELISA at 1:1562500. NEK3 antibody can be used for detection of NEK3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NEK3 antibody at -20 °C.

Details zu NEK3

Target: NEK3 (NIMA related kinase 3 (NEK3))

Andere Bezeichnung: NEK3 (NEK3 Produkte)

Synonyme: HSPK36 antikoerper, ATNEK3 antikoerper, NIMA-RELATED KINASE3 antikoerper, NIMA-related kinase 3 antikoerper, T8M17.60 antikoerper, T8M17_60 antikoerper, NIMA related kinase 3 antikoerper, NIMA (never in mitosis gene a)-related expressed kinase 3 antikoerper, NIMA-related kinase 3 S homeolog antikoerper, NIMA-related kinase 3 antikoerper, NEK3 antikoerper, Nek3 antikoerper, nek3.S antikoerper

Hintergrund: NEK3 is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. It differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase.In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized.

Molekulargewicht: 58 kDa

Gen-ID: 4752

NCBI Accession: NP_002489

UniProt: P51956