Fc epsilon RI/FCER1A Antikörper (Extracellular Domain)

Produktnummer ABIN2451980

Produktdetails anti-Fc epsilon RI/FCER1A Antikörper

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Bindungsspezifität: AA 1-84, Extracellular Domain

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fc epsilon RI/FCER1A Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktmerkmale: This product is the IgG fraction purified from serum-free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions. Properties of CRA1 and CRA2 ntibodies have been extensively characterized by Prof. Ra (Ref. 5, 6). a

Aufreinigung: Purified

Sterilität: Sterile filtered

Immunogen: Recombinant extracellular portion of human FcepsilonR1alpha

Klon: CRA1

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: ~1 g/mL
2) FACS
3) Immunohistochemistry and cytochemistry (paraffin embedding section & frozen section)
4) Titration of IgE-bound receptor in combination with CRA2 antibody
5) Promotion of migration of basophils

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Referenzen für anti-Fc epsilon RI/FCER1A Antikörper (ABIN2451980)

Suzukawa, Hirai, Iikura, Nagase, Komiya, Yoshimura-Uchiyama, Yamada, Ra, Ohta, Yamamoto, Yamaguchi: "IgE- and FcepsilonRI-mediated migration of human basophils." in: International immunology, Vol. 17, Issue 9, pp. 1249-55, (2005) (PubMed).

Takai, Yuuki, Iwamoto-Yasue, Okumura, Ra: "Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions." in: Bioscience, biotechnology, and biochemistry, Vol. 64, Issue 9, pp. 1856-67, (2001) (PubMed).

Takai, Okumura, Ra: "Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli." in: Bioscience, biotechnology, and biochemistry, Vol. 65, Issue 1, pp. 79-85, (2001) (PubMed).

Hasegawa, Pawankar, Suzuki, Nakahata, Furukawa, Okumura, Ra: "Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes." in: Blood, Vol. 93, Issue 8, pp. 2543-51, (1999) (PubMed).

Yamaguchi, Sayama, Yano, Lantz, Noben-Trauth, Ra, Costa, Galli et al.: "IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5455-65, (1999) (PubMed).

Hakimi, Seals, Kondas, Pettine, Danho, Kochan: "The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding." in: The Journal of biological chemistry, Vol. 265, Issue 36, pp. 22079-81, (1991) (PubMed).

Ra, Jouvin, Blank, Kinet: "A macrophage Fc gamma receptor and the mast cell receptor for IgE share an identical subunit." in: Nature, Vol. 341, Issue 6244, pp. 752-4, (1989) (PubMed).

Details zu Fc epsilon RI/FCER1A

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Abstract: FCER1A Produkte

Synonyme: FCER1A antikoerper, FCE1A antikoerper, FcERI antikoerper, Iger01 antikoerper, RATIGER01 antikoerper, Fce1a antikoerper, Fcr-5 antikoerper, fcepsilonri antikoerper, Fc fragment of IgE receptor Ia antikoerper, Fc receptor, IgE, high affinity I, alpha polypeptide antikoerper, FCER1A antikoerper, Fcer1a antikoerper

Hintergrund: FcεR1alpha is subunit of the high affinity receptor for IgE to which IgE directly binds. FcεR1 is a tetrameric complex consisting of one alpha, one beta and two γ subunits. The latter two subunits are required for signal transduction activity. The FcεR1 complex plays an important role in triggering allergic responses. The CRA1 (AER37) monoclonal antibody reacts with the FcεR1alpha subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete with IgE for the receptor binding. Since the CRA2 (AER24) monoclonal antibody reacts with the IgE binding site on FcεR1alpha, it competes with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound FcεR1alpha.

UniProt: P12319

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process