Binds to intact whole C. botulinum Toxin A, amino acids 1280-1292. Has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of C. botulinum Toxin A. Inhibits the entry of C. botulinum Toxin A to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.
Kreuzreaktivität (Details)
Cross-reactivity: C. botulinum Toxin A (1280-1292) 100 % C. botulinum Toxin A 100 % C. botulinum Toxin B 0 % C. botulinum Toxin C 0 % C. botulinum Toxin E 0 % C. botulinum Toxin F (1272-1280) 0 %
Produktmerkmale
MAb to C. botulinum Toxin A, Monoclonal antibody to Clostridium botulinum Toxin A (AA 1280-1292)
Aufreinigung
Supernatant Ascites
Immunogen
Synthetic peptide analogue of the carboxyl terminal region of C. botulinum Toxin A (Catalog ABIN573255)
Suitable for use in ELISA, IFA and RIA. Suitable for use in an indirect immunocytochemical staining procedure at a dilution of 1:25. Prior incubation of the antibody with synthetic peptide immunogen has been shown to eliminate the inhibition of cellular binding by the antibody. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konservierungsmittel
Without preservative
Handhabung
Avoid multiple freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.