ERK2 Antikörper (pThr180, Tyr182)

Produktnummer ABIN220473

Dieses Anti-ERK2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ERK2 in WB, ELISA, IF, IP, ICC und FACS. Geeignet für Human.

Kurzübersicht für ERK2 Antikörper (pThr180, Tyr182) (ABIN220473)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-ERK2 Antikörper

Bindungsspezifität: pThr180, Tyr182

Spezifität: Detects endogenous levels of p38 MAP kinase only when activated by dual phosphorylation at Thr180 and Tyr182. It will also react with p38 singly phosphorylated at Thr180 and singly phosphorylated at Tyr182. Does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species cross-reactivity: Human.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.

Type of Immunogen: Synthetic phospho-peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo (1:100), ICC (1:100), IF, IP (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunofluorescence/ Immunocytochemistry: 1:100. Flow Cytometry: 1:100.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, BSA, 50 % glycerol.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Andere Bezeichnung: MAPK1 / ERK2 / p42

Hintergrund: Name/Gene ID: MAPK1
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK1, ERK, ERK-2, ERK2, MAP kinase 1, MAPK 2, MAPK2, MAP kinase isoform p42, MAPK 1, p41mapk, PRKM2, MAP kinase 2, p41, p42-MAPK, PRKM1, ERT1, p38, p40, p42MAPK, Protein tyrosine kinase ERK2

Gen-ID: 5594

UniProt: P28482

Pathways: MAPK Signalweg, RTK Signalweg, Apoptose, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagie, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteine