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Calprotectin Antikörper (S100A8/A9) (FITC) Primary Antibody

S100A8/A9 Reaktivität: Human HPLC, FACS, WB Wirt: Maus Monoclonal 27E10 FITC
Pubmed (4 Referenzen)
Produktnummer ABIN2192018
$885.41
Zzgl. Versandkosten $45.00
100 μg
Lieferung in 6 bis 8 Werktagen
  • Target Alle Calprotectin (S100A8/A9) Antikörper anzeigen
    Calprotectin (S100A8/A9)
    Reaktivität
    • 27
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 20
    • 8
    Maus
    Klonalität
    • 20
    • 8
    Monoklonal
    Konjugat
    • 13
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Dieser Calprotectin Antikörper ist konjugiert mit FITC
    Applikation
    • 20
    • 12
    • 12
    • 11
    • 6
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    High Pressure Liquid Chromatography (HPLC), Flow Cytometry (FACS), Western Blotting (WB)
    Kreuzreaktivität (Details)
    Cross reactivity: Mouse : No, Rhesus Monkey : Yes (subpopulation of macrophages)
    Sterilität
    0.2 μm filtered
    Klon
    27E10
    Isotyp
    IgG1
  • Applikationshinweise
    For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Human granulocytes control Negative Platelets, lymphocytes, HL-60 cells control
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS, containing 1 % bovine serum albumin and 0.02 % sodium azide.
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Product should be stored at 4 °C Under recommended storage conditions, product is stable for one year.
    Haltbarkeit
    12 months
  • Champaiboon, Sappington, Guenther, Ross, Herzberg: "Calprotectin S100A9 calcium-binding loops I and II are essential for keratinocyte resistance to bacterial invasion." in: The Journal of biological chemistry, Vol. 284, Issue 11, pp. 7078-90, (2009) (PubMed).

    Kuhn, Sonntag, Sunderkötter, Lehmann, Vestweber, Ruzicka: "Upregulation of epidermal surface molecule expression in primary and ultraviolet-induced lesions of lupus erythematosus tumidus." in: The British journal of dermatology, Vol. 146, Issue 5, pp. 801-9, (2002) (PubMed).

    Hessian, Fisher: "The heterodimeric complex of MRP-8 (S100A8) and MRP-14 (S100A9). Antibody recognition, epitope definition and the implications for structure." in: European journal of biochemistry / FEBS, Vol. 268, Issue 2, pp. 353-63, (2001) (PubMed).

    Zwadlo, Schlegel, Sorg: "A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 2, pp. 512-8, (1986) (PubMed).

  • Target
    Calprotectin (S100A8/A9)
    Andere Bezeichnung
    Calprotectin (S100A8/A9 Produkte)
    Synonyme
    60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, Mrp14, MRP-14, p14, TSP-1, TSP1, Thbs-1, tbsp1, 60B8Ag, AW546964, BEE22, Cagb, GAGB, L1Ag, S100 calcium binding protein A9, thrombospondin 1, S100 calcium binding protein A9 (calgranulin B), S100A9, S100a9, Thbs1
    Hintergrund
    The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin- B). The calcium-binding migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60 % of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets. Aliases S100A8/A9, MRP-8/MRP-14, calprotectin, calgranulin-A/calgranulin-B, L1-protein Immunogen Human blood monocytes
    Pathways
    S100 Proteine
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